Highly efficient modification of beta-lactoglobulin (BLG) gene via zinc-finger nucleases in cattle
نویسندگان
چکیده
منابع مشابه
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.
Custom-made zinc-finger nucleases (ZFNs) can induce targeted genome modifications with high efficiency in cell types including Drosophila, C. elegans, plants, and humans. A bottleneck in the application of ZFN technology has been the generation of highly specific engineered zinc-finger arrays. Here we describe OPEN (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constr...
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Agricultural biotechnology is limited by the inefficiency and unpredictability of conventional random mutagenesis and transgenesis. We have developed a broadly applicable, versatile alternative: the use of designed zinc-finger nucleases (ZFNs) that induce double-stranded breaks (DSB) at their target locus. We show that ZFNs designed to cleave an endogenous maize gene disrupt their target via DS...
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Homologous recombination-based gene targeting using Mus musculus embryonic stem cells has greatly impacted biomedical research. This study presents a powerful new technology for more efficient and less time-consuming gene targeting in mice using embryonic injection of zinc-finger nucleases (ZFNs), which generate site-specific double strand breaks, leading to insertions or deletions via DNA repa...
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Genetically engineered zinc-finger nucleases (ZFNs) are useful for marker-free gene targeting using a one-step approach. We used ZFNs to efficiently disrupt bovine myostatin (MSTN), which was identified previously as the gene responsible for double muscling in cattle. The mutation efficiency of bovine somatic cells was approximately 20%, and the biallelic mutation efficiency was 8.3%. To evalua...
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ژورنال
عنوان ژورنال: Cell Research
سال: 2011
ISSN: 1001-0602,1748-7838
DOI: 10.1038/cr.2011.153